Characterization of iron-sulfur clusters in flavin-containing opine dehydrogenase*. Issue 12 (1st December 2016)

• Record Type: Journal Article
• Authors: Watanabe, Seiya; Tajima, Kunihiko; Matsui, Kazuma; Watanabe, Yasuo
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 12(2016)
• Page Start: 2371
• Page End: 2375
• Publication Date: 2016-12-01
• Subjects: opine dehydrogenase -- [4Fe-4S] cluster -- [2Fe-2S] cluster -- electron spin resonance spectroscopy; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2016.1206812
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract : Flavin-containing opine dehydrogenase from Bradyrhizobium japonicum forms a heterooligomeric α4 β4 γ4 enzyme complex. An electron paramagnetic resonance spectroscopy analysis using wild-type and site-directed mutants revealed that [4Fe-4S] and [2Fe-2S] clusters bind to two different types of [Fe-S] binding sites in the γ- and α-subunits, respectively. The latter was found to be important for structural folding and enzyme catalysis.