Development and evaluation of an experimental protocol for 3-D visualization and characterization of the structure of bacterial biofilms in porous media using laboratory X-ray tomography. Issue 10 (25th November 2016)

• Record Type: Journal Article
• Authors: Ivankovic, Tomislav; Rolland du Roscoat, Sabine; Geindreau, Christian; Séchet, Philipe; Huang, Zhujun; Martins, Jean M. F.
• Is Part Of: Biofouling. Volume 32:Issue 10(2016)
• Page Start: 1235
• Page End: 1244
• Publication Date: 2016-11-25
• Subjects: Biofilter -- biofilm -- X-ray tomography -- Pseudomonas putida; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2016.1249865
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: The development of a reliable model allowing accurate predictions of biofilm growth in porous media relies on a good knowledge of the temporal evolution of biofilm structure within the porous network. Since little is known about the real 3-D structure of biofilms in porous media, this work was aimed at developing a new experimental protocol to visualize the 3-D microstructure of the inside of a porous medium using laboratory X-ray microtomography. A reliable and reproducible methodology is proposed for (1) growing a biofilm inside a porous medium, and (2) X-ray tomography-based characterization of the temporal development of the biofilm at the inlet of the biofilter. The statistical analysis proposed here also validates the results presented in the literature based on a biofilm structure single measurement.