Effects of repeated high-dose methamphetamine and ceftriaxone post-treatments on tissue content of dopamine and serotonin as well as glutamate and glutamine. (10th November 2016)

• Record Type: Journal Article
• Authors: Althobaiti, Yusuf S.; Almalki, Atiah H.; Das, Sujan C.; Alshehri, Fahad S.; Sari, Youssef
• Is Part Of: Neuroscience letters. Volume 634(2016)
• Page Start: 25
• Page End: 31
• Publication Date: 2016-11-10
• Subjects: Methamphetamine -- Dopamine -- Serotonin -- Glutamate -- Glutamine -- Ceftriaxone; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2016.09.058
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Methamphetamine caused depletion of dopamine and 5-HT in NAc and PFC. Dopamine tissue content was restored with ceftriaxone post-treatment in NAc. Methamphetamine decreased glutamate and glutamine tissue content in PFC. Ceftriaxone attenuated the effect of methamphetamine on glutamate and glutamine. Abstract: Repeated exposure to high doses of methamphetamine (METH) is known to alter several neurotransmitters in certain brain regions. Little is known about the effects of ceftriaxone (CEF), a β-lactam antibiotic, known to upregulate glutamate transporter subtype 1, post-treatment on METH-induced depletion of dopamine and serotonin (5-HT) tissue content in brain reward regions. Moreover, the effects of METH and CEF post-treatment on glutamate and glutamine tissue content are not well understood. In this study, Wistar rats were used to investigate the effects of METH and CEF post-treatment on tissue content of dopamine/5-HT and glutamate/glutamine in the nucleus accumbens (NAc) and prefrontal cortex (PFC). Rats received either saline or METH (10 mg/kg, i.p. every 2 h × 4) followed by either saline or CEF (200 mg/kg, i.p, every day × 3) post-treatment. METH induced a significant depletion of dopamine and 5-HT in the NAc and PFC. Importantly, dopamine tissue content was completely restored in the NAc following CEF post-treatment. Additionally, METH caused a significant decrease in glutamate and glutamine tissue content in PFC, and this effect was attenuated by CEF post-treatment. These findings demonstrate for the first time the attenuating effects of CEF post-treatment on METH induced alterations in the tissue contents of dopamine, glutamate, and glutamine.