High intensity interval exercise enhances the global HDAC activity in PBMC and anti-inflammatory cytokines of overweight-obese subjects. (June 2016)

• Record Type: Journal Article
• Authors: Dorneles, Gilson Pires; da Silva, Ivy Reichert Vital; Korb, Arthiese; Bertoldi, Karine; Siqueira, Ionara Rodrigues; Elsner, Viviane Rostirola; Romão, Pedro Roosevelt Torres; Peres, Alessandra
• Is Part Of: Obesity medicine. Volume 2(2016)
• Page Start: 25
• Page End: 30
• Publication Date: 2016-06
• Subjects: Histone deacetylase -- Epigenetic modulation -- Cytokine -- Inflammation -- Interval exercise -- Obesity; Obesity -- Periodicals; Obesity; Obesity; Periodicals; Periodicals; 616.398005
• DOI: 10.1016/j.obmed.2016.05.004
• Languages: English
• ISSNs: 2451-8476

Abstract

Abstract: Objective: To evaluate the global activity of histone deacetylases (HDACs) in peripheral blood mononuclear cells (PBMC) and systemic levels of cytokines in response to high intensity interval exercise (HIIE) in overweight-obese individuals. Methods: In order to verify the acute effects of exercise on epigenetic and inflammatory modulation, ten overweight-obese males (BMI >25 to <35 kg/m 2 ) performed a single bout of HIIE (10 bouts of 60 s to 85–90%PMax /75 s to 50%PMax ) and the blood samples were collected pre, immediately post and 24 h post HIIE session for cytokine measurements and HDAC activity analyses. The global activity of HDAC in PBMC was determined by fluorometric assay and the serum concentrations of IL-6, IL-10, IL-17a, TGF-β and TNF-α were quantified by ELISA. Results: HIIE session induced a leukocytosis (27%, p = 0.001), characterized by the increase in the number of circulating lymphocytes (41%, p = 0.002) and monocytes (59%, p = 0.001). Interestingly, a significant increase in global HDAC activity in PBMC (132%, p = 0.002) with a concomitant increase in serum concentrations of IL-6 (11%, p = 0.04), IL-10 (27%, p = 0.003) and TGF-β (63.44%, p = 0.05) were observed immediately after exercise. At this time, no significant difference was observed in the levels of TNF-α and IL-17a. None of these variables was significantly changed at 24 h after HIIE. Conclusion: These data support the hypothesis that the anti-inflammatory effects of exercise may be related, at least in part, to the modulation of HDAC activity in PBMC of individuals with overweight-obese. Highlights: Obese individuals exhibit a low grade systemic inflammatory profile. High intensity interval exercise (HIIE) increased HDAC activity in PBMC of obese. HIIE increased systemic levels of IL-6, IL-10 an TGF-β in obese individuals. HIIE may help to reduce low grade inflammation in obesity through epigenetic modulation of leukocytes.