In vitro and in vivo assessment of meadowsweet (Filipendula ulmaria) as anti-inflammatory agent. (4th December 2016)

• Record Type: Journal Article
• Authors: Katanić, Jelena; Boroja, Tatjana; Mihailović, Vladimir; Nikles, Stefanie; Pan, San-Po; Rosić, Gvozden; Selaković, Dragica; Joksimović, Jovana; Mitrović, Slobodanka; Bauer, Rudolf
• Is Part Of: Journal of ethnopharmacology. Volume 193(2016)
• Page Start: 627
• Page End: 636
• Publication Date: 2016-12-04
• Subjects: FUA F. ulmaria aerial part extract -- FUR F. ulmaria root extract -- COX-1 cyclooxygenase-1 -- COX-2 cyclooxygenase-2 -- LOX lipoxygenase -- NSAIDs nonsteroidal anti-inflammatory drugs -- HPTLC high performance thin layer chromatography -- HPLC high performance liquid chromatography -- IL interleukin -- dex dexamethasone -- NFκB nuclear factor kappa B -- TNF-α tumor necrosis factor alpha -- PAF platelet-activating factor -- PPAR α and γ peroxisome proliferator-activated receptors; hyperoside (PubChem CID: 5281643) -- rutoside (PubChem CID: 5280805) -- apigenin (PubChem CID: 5280443) -- spiraeoside (PubChem CID: 5320844) -- quercetin (PubChem CID: 5280343) -- isoquercitrin (PubChem CID: 5280804) -- indomethacin (PubChem CID: 3715) -- NS-398 (PubChem CID: 4553) -- dexamethasone (PubChem CID: 5743) -- λ-carrageenan (PubChem CID: 91972149); Filipendula ulmaria -- Inflammation -- Cyclooxygenase -- Hot plate test -- Carrageenan-induced edema; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.10.015
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Meadowsweet ( Filipendula ulmaria (L.) Maxim, Rosaceae) has been traditionally used in most European countries for the treatment of inflammatory diseases due to its antipyretic, analgesic, astringent, and anti-rheumatic properties. However, there is little scientific evidence on F. ulmaria anti-inflammatory effects regarding its impact on cyclooxygenases enzymatic activity and in vivo assessment of anti-inflammatory potential. This study aims to reveal the anti-inflammatory activity of methanolic extracts from the aerial parts (FUA) and roots (FUR) of F. ulmaria, both in in vitro and in vivo conditions. Materials and methods: The characteristic phenolic compounds in F. ulmaria extracts were monitored via high performance thin layer chromatography (HPTLC). The in vitro anti-inflammatory activity of F. ulmaria extracts was evaluated using cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzyme assays, and an assay for determining COX-2 gene expression. The in vivo anti-inflammatory effect of F. ulmaria extracts was determined in two doses (100 and 200 mg/kg b.w.) with hot plate test and carrageenan-induced paw edema test in rats. Inflammation was also evaluated by histopathological and immunohistochemical analysis. Results: FUA extract showed the presence of rutoside, spiraeoside, and isoquercitrin. Both F. ulmaria extracts at a concentration of 50 μg/mL were able to inhibit COX-1 and -2 enzyme activities, whereby FUA extract (62.84% and 46.43% inhibition, respectively) was double as effective as the root extract (32.11% and 20.20%, respectively). Extracts hardly inhibited the level of COX-2 gene expression in THP-1 cells at a concentration of 25 μg/mL (10.19% inhibition by FUA and 8.54% by FUR). In the hot plate test, both extracts in two doses (100 and 200 mg/kg b.w.), exhibited an increase in latency time when compared with the control group ( p <0.05). In the carrageenan-induced acute inflammation test, FUA at doses of 100 and 200 mg/kg b.w., and FUR at 200 mg/kg, were able to significantly reduce the mean maximal swelling of rat paw until 6 h of treatment. Indomethacin, FUA, and FUR extracts significantly decreased inflammation score and this effect was more pronounced after 24 h, compared to the control group ( p <0.05). Conclusions: The observed results of in vitro and, for the first time, in vivo anti-inflammatory activity of meadowsweet extracts, provide support of the traditional use of this plant in the treatment of different inflammatory conditions. Further investigation of the anti-inflammatory compounds could reveal the mechanism of anti-inflammatory action of these extracts. Graphical abstract: