Related structures of neutral capsular polysaccharides of Acinetobacter baumannii isolates that carry related capsule gene clusters KL43, KL47, and KL88. (29th November 2016)

• Record Type: Journal Article
• Authors: Shashkov, Alexander S.; Kenyon, Johanna J.; Arbatsky, Nikolay P.; Shneider, Mikhail M.; Popova, Anastasiya V.; Miroshnikov, Konstantin A.; Hall, Ruth M.; Knirel, Yuriy A.
• Is Part Of: Carbohydrate research. Volume 435(2016)
• Page Start: 173
• Page End: 179
• Publication Date: 2016-11-29
• Subjects: Acinetobacter baumannii -- Capsular polysaccharide -- K locus -- Glycosyltransferase -- Wzy polymerase; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2016.10.007
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Capsular polysaccharides were recovered from four Acinetobacter baumannii isolates, and the following related structures of oligosaccharide repeating units were established by sugar analyses along with 1D and 2D 1 H and 13 C NMR spectroscopy: NIPH 60 and LUH5544 (K43) NIPH 601 (K47) The K locus for capsule biosynthesis in the genome sequences available for NIPH 60 and LUH5544, designated KL43, was found to be related to gene clusters KL47 in NIPH 601 and KL88 in LUH5548. The three clusters share most gene content differing in only a small portion that includes an additional glycosyltransferase genes in KL47 and KL88, as well as genes encoding distinct Wzy polymerases that were found to form the same α-d -Glc p NAc-(1 → 6)-α-d -Glc p NAc linkage in K43 and K47. Graphical abstract: Highlights: Capsular polysaccharide structures of four A. baumannii isolates were elucidated. Repeating units of the capsular polysaccharides studied share a trisaccharide fragment. Gene clusters for capsule biosynthesis of the strains studied are related.