Structural features of N-glycans linked to glycoproteins expressed in three kinds of water plants: Predominant occurrence of the plant complex type N-glycans bearing Lewis a epitope. (29th November 2016)

• Record Type: Journal Article
• Authors: Maeda, Megumi; Tani, Misato; Yoshiie, Takeo; Vavricka, Christopher J.; Kimura, Yoshinobu
• Is Part Of: Carbohydrate research. Volume 435(2016)
• Page Start: 50
• Page End: 57
• Publication Date: 2016-11-29
• Subjects: Plant complex type N-Glycans -- Lewis a epitope -- Water plants; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2016.09.008
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The Japanese cedar pollen allergen (Cry j1) and the mountain cedar pollen allergen (Jun a1) are glycosylated with plant complex type N -glycans bearing Lewis a epitope(s) (Galβ1-3[Fucα1-4]GlcNAc-). The biological significance of Lewis a type plant N -glycans and their effects on the human immune system remain to be elucidated. Since a substantial amount of such plant specific N -glycans are required to evaluate immunological activity, we have searched for good plant-glycan sources to characterize Lewis a epitope-containing plant N -glycans. In this study, we have found that three water plants, Elodea nuttallii, Egeria densa, and Ceratophyllum demersum, produce glycoproteins bearing Lewis a units. Structural analysis of the N -glycans revealed that almost all glycoproteins expressed in these three water plants predominantly carry plant complex type N -glycans including the Lewis a type, suggesting that these water plants are good sources for preparation of Lewis a type plant N -glycans in substantial amounts. Graphical abstract: Highlights: Lewis a type plant N -glycans are found in glycoproteins of three water plants. The Lewis a N -glycans have the same structure as those of cedar pollen allergens. Water plants are a good source of Lewis a N -glycans to reveal the immune responses.