Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study. Issue 10 (8th September 2016)

• Record Type: Journal Article
• Authors: Nishio, N; Fujimoto, Y; Suga, K; Iwata, Y; Toriyama, K; Takanari, K; Kamei, Y; Yamamoto, T; Gotoh, M
• Is Part Of: Journal of laryngology & otology. Volume 130:Issue 10(2016)
• Page Start: 914
• Page End: 922
• Publication Date: 2016-09-08
• Subjects: Stem Cells, -- Injections, -- Vocal Cord Paralysis, -- Animal, -- Hoarseness; Otolaryngology -- Periodicals; 617.51
• DOI: 10.1017/S0022215116008707
• Languages: English
• ISSNs: 0022-2151

Abstract

Abstract: Objectives: To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy. Methods: Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted. Results: Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site. Conclusion: The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.