Functional exhaustion of CD4+ T cells induced by co‐stimulatory signals from myeloid leukaemia cells. Issue 4 (29th September 2016)

• Record Type: Journal Article
• Authors: Ozkazanc, Didem; Yoyen‐Ermis, Digdem; Tavukcuoglu, Ece; Buyukasik, Yahya; Esendagli, Gunes
• Is Part Of: Immunology. Volume 149:Issue 4(2016)
• Page Start: 460
• Page End: 471
• Publication Date: 2016-09-29
• Subjects: cancer -- helper T cell -- interleukin‐2 -- immune escape -- lymphocyte activation gene 3 -- programmed cell death 1 -- T‐cell immunoglobulin and mucin domain‐containing protein‐3; Immunology -- Periodicals
• DOI: 10.1111/imm.12665
• Languages: English
• ISSNs: 0019-2805

Abstract

Summary: To cope with immune responses, tumour cells implement elaborate strategies such as adaptive resistance and induction of T‐cell exhaustion. T‐cell exhaustion has been identified as a state of hyporesponsiveness that arises under continuous antigenic stimulus. Nevertheless, contribution of co‐stimulatory molecules to T‐cell exhaustion in cancer remains to be better defined. This study explores the role of myeloid leukaemia‐derived co‐stimulatory signals on CD4 + T helper (Th) cell exhaustion, which may limit anti‐tumour immunity. Here, CD86 and inducible T‐cell co‐stimulator ligand (ICOS‐LG) co‐stimulatory molecules that are found on myeloid leukaemia cells supported Th cell activation and proliferation. However, under continuous stimulation, T cells co‐cultured with leukaemia cells, but not with peripheral blood monocytes, became functionally exhausted. These in vitro ‐generated exhausted Th cells were defined by up‐regulation of programmed cell death 1 (PD‐1), cytotoxic T‐lymphocyte antigen 4 (CTLA‐4), lymphocyte activation gene 3 (LAG3) and T‐cell immunoglobulin and mucin domain‐containing protein 3 (TIM‐3) inhibitory receptors. They were reluctant to proliferate upon re‐stimulation and produced reduced amounts of interleukin‐2 (IL‐2), tumour necrosis factor‐ α (TNF‐ α ) and interferon‐ γ (IFN‐ γ ). Nonetheless, IL‐2 supplementation restored the proliferation capacity of the exhausted Th cells. When the co‐stimulation supplied by the myeloid leukaemia cells were blocked, the amount of exhausted Th cells was significantly decreased. Moreover, in the bone marrow aspirates from patients with acute myeloid leukaemia (AML) or myelodysplastic syndrome (MDS), a subpopulation of Th cells expressing PD‐1, TIM‐3 and/or LAG3 was identified together with CD86 + and/or ICOS‐LG + myeloid blasts. Collectively, co‐stimulatory signals derived from myeloid leukaemia cells possess the capacity to facilitate functional exhaustion in Th cells. Abstract : Potent co‐stimulatory molecules (CD86 and ICOS‐LG) expressed by myeloid leukaemia cells promote CD4 + helper T (Th) cell responses. Extended exposure to co‐stimulation facilitates a state of functional hyporesponsiveness in Th cells. This study demonstrates the role of myeloid leukaemia‐derived co‐stimulatory signals on Th cell exhaustion, which may limit anti‐tumour immunity.