MicroRNA‐463‐3p/ABCG4: A new axis in glucose‐stimulated insulin secretion. Issue 11 (24th September 2016)

• Record Type: Journal Article
• Authors: Hou, Xuwei; Wu, Wei; Yin, Bo; Liu, Xuefeng; Ren, Fu
• Is Part Of: Obesity. Volume 24:Issue 11(2016)
• Page Start: 2368
• Page End: 2376
• Publication Date: 2016-09-24
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.21655
• Languages: English
• ISSNs: 1930-7381

Abstract

Abstract : Objective: Glucose‐stimulated insulin secretion (GSIS) is known to be essential in the control of metabolic fuel homeostasis, though the molecular mechanisms involved remain unclear. Methods: MicroRNA (miRNA)−463‐3p and ATP‐binding cassette A4 (ABCG4) expression was analyzed by real‐time PCR, and the potential role of miRNA‐463‐3p or ABCG4 was evaluated by overexpressing or silencing such miRNA or genes. Results: The miRNA‐463‐3p inhibited GSIS without affecting cell viability. Further, mechanistic studies demonstrated that ABCG4 was a direct target of microRNA‐463‐3p and, to this effect, that ABCG4 played an important role in GSIS. The targeting was relevant in pancreatic islet β‐cells, where GSIS through the miRNA‐463‐3p/ABCG4 axis was observed. Interestingly, in type 2 diabetes human pancreatic islets, expression of miRNA‐463‐3p and insulin was upregulated and ABCG4 downregulated compared with nondiabetic controls, and their expression levels were closely correlated. Conclusions: The findings collectively establish a link between GSIS and the miRNA‐463‐3p/ABCG4 axis and represent a promising target for future diabetes mellitus treatments.