Cover Picture: DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity (ChemBioChem 21/2016). (26th October 2016)

• Record Type: Journal Article
• Authors: Cox, Amanda J.; Bengtson, Hillary N.; Gerasimova, Yulia V.; Rohde, Kyle H.; Kolpashchikov, Dmitry M.
• Is Part Of: Chembiochem. Volume 17:Number 21(2016)
• Page Start: 1995
• Page End: 1995
• Publication Date: 2016-10-26
• Subjects: deoxyribozymes -- diffusion-limited reactions -- DNA crossover tile -- mycobacterium tuberculosis -- split hybridization probes; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201600547
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : The cover picture shows a molecular antenna tile made of DNA that attracts a fluorophore and a quencher (labeled Fluorogenic Substrate) from solution, thus increasing its local concentration at the tile. In the middle of the tile, there is a deoxyribozyme (DZ) sensor activated by binding to a specific DNA or RNA analyte (e.g., RNA of Mycobacterium tuberculosis ). The catalytically active DZ sensor cleaves multiple fluorogenic substrate molecules, thus producing bright fluorescence. Lower limits of detection can be achieved with this design owing to the enhanced substrate delivery to the DZ catalytic core. More information can be found in the communication by D. M. Kolpashchikov et al. onpage 2038 in Issue 21, 2016 (DOI:10.1002/cbic.201600438 ).