DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity. (13th September 2016)

• Record Type: Journal Article
• Authors: Cox, Amanda J.; Bengtson, Hillary N.; Gerasimova, Yulia V.; Rohde, Kyle H.; Kolpashchikov, Dmitry M.
• Is Part Of: Chembiochem. Volume 17:Number 21(2016)
• Page Start: 2038
• Page End: 2041
• Publication Date: 2016-09-13
• Subjects: deoxyribozymes -- diffusion-limited reactions -- DNA crossover tile -- mycobacterium tuberculosis -- split hybridization probes; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201600438
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Some natural enzymes increase the rate of diffusion‐limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three‐ to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion‐limited enzymes and to improve sensitivity of enzyme‐based biosensors. Abstract : Time for tiles : Deoxyribozyme (DZ) sensors for fluorescent detection of nucleic acids demonstrated improved sensitivities and limits of detection when attached to a DNA tile, which attracted and increased the local concentration of the fluorogenic reporter substrate. This strategy can be used to enhance catalytic efficiency of diffusion‐limited enzymes and improve sensitivity of enzyme‐based biosensors.