Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity. (December 2016)

• Record Type: Journal Article
• Authors: Carvalho, Dália R.A.; Vasconcelos, Marta W.; Lee, Sangseok; Koning-Boucoiran, Carole F.S.; Vreugdenhil, Dick; Krens, Frans A.; Heuvelink, Ep; Carvalho, Susana M.P.
• Is Part Of: Plant science. Volume 253(2016:Dec.)
• Page Start: 154
• Page End: 163
• Publication Date: 2016-12
• Subjects: AAO abscisic aldehyde oxidase -- ABA-GE abscisic acid-β-d-glucosyl ester -- ABF abscisic acid-responsive element-binding factor -- APX ascorbate peroxidase -- BG1 β-glucosidase homolog1 -- BG2 β-glucosidase homolog2 -- DPA 4′-dihydrophaseic acid -- DREB dehydration responsive element binding -- gs stomatal conductance -- NCED 9-cis epoxycarotenoid dioxygenase -- OST1 Open stomata 1 -- PA phaseic acid -- RGE relative gene expression -- RH relative air humidity -- RWC_4 h relative water content after four hours of leaflet desiccation -- UGT uridine diphosphate glucosyltransferase -- VPD vapor pressure deficit -- 10_D 10 min after the beginning of the dark period -- 180_D 180 min after the beginning of the dark period -- 10_L 10 min after the beginning of the light period -- 180_L 180 min after the beginning of the light period; Abscisic acid -- Stomatal anatomy -- Tetraploid roses -- Vapor pressure deficit -- Water loss; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2016.09.018
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: High RH reduced leaf [ABA] more in the sensitive genotype than in the tolerant one Several genes acted together towards genotypic tolerance to high RH. NCED1, UGT75B2, BG2, OST1, ABF3 and RhAPX seem to be involved in tolerance to high RH. CYP707A3 and BG1 had a minor contribution to tolerance to high RH. AAO3, CYP707A1 and DREB1B did not contribute to this genotypic tolerance trait. Abstract: High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs ) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning ( NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX ) while two others showed a minor contribution ( CYP707A3 and BG1 ) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.