Acute phenanthrene toxicity to juvenile diploid and triploid African catfish (Clarias gariepinus): Molecular, biochemical, and histopathological alterations. (May 2016)

• Record Type: Journal Article
• Authors: Karami, Ali; Romano, Nicholas; Hamzah, Hazilawati; Simpson, Stuart L.; Yap, Chee Kong
• Is Part Of: Environmental pollution. Volume 212(2016)
• Page Start: 155
• Page End: 165
• Publication Date: 2016-05
• Subjects: Polyploidy -- Fish -- Biomarkers -- Biochemical -- Gene expressions -- Histopathology; Phe phenanthrene -- TP total protein -- AST aspartate aminotransferase -- ALT alanine aminotransferase -- LDH lactate dehydrogenase -- ALP alkaline phosphatase -- γGT gamma-glutamyl transpeptidase -- HDL high-density lipoprotein -- LDL low-density lipoprotein -- GnRH gonadotropin-releasing hormone -- tph2 tryptophan hydroxylase2 -- cyp19a2 brain aromatase -- foxl2 forkhead box L2 -- ftz-f1 fushi tarazu-factor 1 -- 11β-hsd2 11 β-hydroxysteroid dehydrogenase type 2; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2016.01.055
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Information on the biological responses of polyploid animals towards environmental contaminants is scarce. This study aimed to compare reproductive axis-related gene expressions in the brain, plasma biochemical responses, and the liver and gill histopathological alterations in diploid and triploid full-sibling juvenile African catfish ( Clarias gariepinus ). Fish were exposed for 96 h to one of the two waterborne phenanthrene (Phe) concentrations [mean measured (SD): 6.2 (2.4) and 76 (4.2) μg/L]. In triploids, exposure to 76 μg/L Phe increased mRNA level of f ushi tarazu-factor 1 ( ftz-f1 ). Expression of tryptophan hydroxylase2 ( tph2 ) was also elevated in both ploidies following the exposure to 76 μg/L Phe compared to the solvent control. In triploids, 76 μg/L Phe increased plasma alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels compared to the other Phe-exposed group. It also elevated lactate and glucose contents relative to the other groups. In diploids, however, biochemical biomarkers did not change. Phenanthrene exposures elevated glycogen contents and the prevalence of histopathological lesions in the liver and gills of both ploidies. This study showed substantial differences between diploids and triploids on biochemical and molecular biomarker responses, but similar histopathological alterations following acute Phe exposures. Highlights: Diploid and triploid African catfish ( Clarias gariepinus) were acutely exposed to two phenanthrene (Phe) concentrations. Substantial differences on some plasma biochemical and brain molecular biomarkers between Phe-exposed ploidies were shown. Similar histopathological changes between ploidies and Phe concentrations were observed. Abstract : Significant differences on molecular and biochemical responses but similar histopathological endpoints between diploid and triploid Clarias gariepinus were shown.