L-Asparaginase Purification. Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Tundisi, Louise L.; Coêlho, Diego F.; Zanchetta, Beatriz; Moriel, Patricia; Pessoa, Adalberto; Tambourgi, Elias B.; Silveira, Edgar; Mazzola, Priscila G.
• Is Part Of: Separation and purification reviews. Volume 46:Issue 1(2017)
• Page Start: 35
• Page End: 43
• Publication Date: 2017-01-02
• Subjects: Aqueous-two phase system -- biotechnology -- downstream process -- L-asparaginase -- purification; Separation (Technology) -- Periodicals; Chemicals -- Purification -- Periodicals; 660.2842
• DOI: 10.1080/15422119.2016.1184167
• Languages: English
• ISSNs: 1542-2119

Abstract

Abstract : L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase.