Mycotoxin contamination in laboratory rat feeds and their implications in animal research. (1st September 2016)

• Record Type: Journal Article
• Authors: Escrivá, Laura; Font, Guillermina; Berrada, Houda; Manyes, Lara
• Is Part Of: Toxicology mechanisms and methods. Volume 26:Number 7(2016)
• Page Start: 529
• Page End: 537
• Publication Date: 2016-09-01
• Subjects: Experimental animals -- feed -- LC-MS/MS -- method validation -- mycotoxins; Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.1080/15376516.2016.1206163
• Languages: English
• ISSNs: 1537-6516

Abstract

Abstract: Compound feed is particularly vulnerable to multi-mycotoxin contamination. A method for the determination of 12 mycotoxins; enniatins A, A1, B, B1; aflatoxins B1, B2, G1, G2; OTA; ZEA; T-2 and HT-2 by liquid chromatography-tandem mass spectrometry has been developed and applied for the analysis of laboratory rat commercial feeds. The method trueness was checked by recovery assays at three different spiked levels ( n = 9). Recoveries ranged from 73% to 112%, and the intra-day and inter-day precision were lower than 9% and 13%, respectively. Limits of quantitation were lower than 15 μg/kg. Twenty-seven laboratory rats feed samples showed multi-contamination by at least three up to six different mycotoxins. ENNs B and B1, followed by ZEA were the most prevalent mycotoxins. T-2, HT-2, and OTA were not detected. ZEA showed the highest concentration levels reaching 492 μg/kg. The results underline the importance of implementing mycotoxin regular surveillance programs for laboratory animal feeds.