Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery. Issue 9 (1st September 2016)

• Record Type: Journal Article
• Authors: Shi, Jian; Li, Yuan; Liang, Shuzhen; Zeng, Jianying; Liu, Guifeng; Mu, Feng; Li, Haibo; Chen, Jibing; Liu, Tongjun; Niu, Lizhi
• Is Part Of: Cancer biology & therapy. Volume 17:Issue 9(2016)
• Page Start: 935
• Page End: 942
• Publication Date: 2016-09-01
• Subjects: Circulating tumor cells -- cryosurgery -- colorectal cancer -- flow cytometry -- liver metastasis -- reverse transcriptase polymerase chain reaction; 616.99406
• DOI: 10.1080/15384047.2016.1210731
• Languages: English
• ISSNs: 1538-4047

Abstract

ABSTRACT: In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time ( P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation ( P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.