Development of quantitative mass spectrometric immunoassay for serum amyloid A. (16th November 2016)

• Record Type: Journal Article
• Authors: Trenchevska, Olgica; Yassine, Hussein N.; Borges, Chad R.; Nelson, Randall W.; Nedelkov, Dobrin
• Is Part Of: Biomarkers. Volume 21:Number 8(2016)
• Page Start: 743
• Page End: 751
• Publication Date: 2016-11-16
• Subjects: Baseline concentration -- inflammation biomarker -- mass spectrometry -- polymorphism -- posttranslational modifications -- proteoforms; Biochemical markers -- Periodicals; 610.28
• DOI: 10.1080/1354750X.2016.1201533
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Objective: Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states. Methods: We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms. Results: Intra- and inter-day precision revealed CVs <10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman–Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples revealed 7 diverse SAA polymorphic types and 12 different proteoforms. Conclusion: The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.