A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer. (20th October 2016)

• Record Type: Journal Article
• Authors: Eftekhari-Sis, Bagher; Karaminejad, Sajede; Karimi, Farrokh
• Is Part Of: Cancer investigation. Volume 34:Number 9(2016)
• Page Start: 431
• Page End: 439
• Publication Date: 2016-10-20
• Subjects: Breast cancer -- graphene oxide -- 185delAG mutation -- nano-biosensor -- fluorescent; Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.1080/07357907.2016.1227444
• Languages: English
• ISSNs: 0735-7907

Abstract

ABSTRACT: A method was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5′ terminal in the presence of cDNA or mDNA was applied.