Assessment of the role of thymol in combating chromium (VI)-induced oxidative stress in isolated rat erythrocytes in vitro. Issue 10 (25th November 2016)

• Record Type: Journal Article
• Authors: Abd-Elhakim, Yasmina M.; Mohamed, Wafaa A.M.
• Is Part Of: Toxicological and environmental chemistry. Volume 98:Issue 10(2016)
• Page Start: 1227
• Page End: 1240
• Publication Date: 2016-11-25
• Subjects: chromium -- isolated rat erythrocytes -- thymol -- osmotic fragility -- oxidative stress; Toxicology -- Periodicals; Environmental chemistry -- Periodicals; 571.95
• DOI: 10.1080/02772248.2015.1121490
• Languages: English
• ISSNs: 0277-2248

Abstract

Abstract : Thymol, the main phenolic compound in Thymus vulgaris, has been shown to have various biological effects. The main objective of this study was to investigate the efficacy of thymol on counteracting hexavalent chromium-induced oxidative damage in rat erythrocytes in vitro . The radical scavenging activity of thymol was examined using the 1, 1-diphenyl-2-picrylhydrazyl assay. Erythrocytes resistance to oxidative damage, lipid peroxidation, osmotic pressure, hemolysis as well as morphological alterations were evaluated in the presence of 2.5 µg thymol mL −1 with or without 5 µmol hexavalent chromium mL −1 of the incubation media. Results from the 1, 1-diphenyl-2-picrylhydrazyl assay denoted good radical scavenging activity of thymol. Thymol caused a significant increase in superoxide dismutase and catalase activities and reduced glutathione content in erythrocytes intoxicated with hexavalent chromium. In contrast, the presence of thymol resulted in markedly less-elevated malondialdehyde levels, hemolysis, and destabilization of erythrocytes exposed to hexavalent chromium. Microscopically, thymol markedly reduced hexavalent chromium-induced morphological alterations in rat red blood cells. Conclusively, thymol counteracted hexavalent chromium-induced oxidative damage in rat erythrocytes.