Rapid and specific detection of porcine parvovirus by isothermal recombinase polymerase amplification assays. Issue 5 (October 2016)

• Record Type: Journal Article
• Authors: Yang, Yang; Qin, Xiaodong; Zhang, Wei; Li, Yanmin; Zhang, Zhidong
• Is Part Of: Molecular and cellular probes. Volume 30:Issue 5(2016)
• Page Start: 300
• Page End: 305
• Publication Date: 2016-10
• Subjects: Recombinase polymerase amplification assay -- Real-time RPA assay -- RPA LFD assay -- PPV; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2016.08.011
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Porcine parvovirus (PPV) is a major cause of swine reproductive failure and reported in many countries worldwide. Recombinase polymerase amplification (RPA) assays using a real-time fluorescent detection (PPV real-time RPA assay) and a lateral flow dipstick (PPV RPA LFD assay) were developed targeting PPV NS1 gene. The detection limit of PPV real-time RPA assay was 300 copies per reaction within 9 min at 38 °C, while the RPA LFD assay has a detection limit of 400 copies per reaction in less than 20 min at 38 °C. In both assays, there were no cross-reactions with porcine circovirus type 2, pseudorabies virus, porcine reproductive and respiratory syndrome virus, classical swine fever virus, and foot-and-mouth disease virus. Based on a total of 128 clinical samples examined, the sensitivity and the specificity of the developed RPA assays for identification of PPV was 94.4% and 100%, respectively, when compared to real-time (qPCR) assay. Therefore, the RPA assay provides a rapid, sensitive and specific alternative for PPV detection. Highlights: PPV real-time RPA assay was developed first time to detect PPV. PPV RPA LFD assay was developed first time to detect PPV. The RPA assay provides a rapid, sensitive and specific alternative for detection of PPV.