NMR Reveals the Interplay among the AMSH SH3 Binding Motif, STAM2, and Lys63-Linked Diubiquitin. Issue 22 (6th November 2016)

• Record Type: Journal Article
• Authors: Hologne, Maggy; Cantrelle, François-Xavier; Riviere, Gwladys; Guillière, Florence; Trivelli, Xavier; Walker, Olivier
• Is Part Of: Journal of molecular biology. Volume 428:Issue 22(2016:Nov. 06)
• Page Start: 4544
• Page End: 4558
• Publication Date: 2016-11-06
• Subjects: AMSH associated molecule with the SH3 domain of STAM -- AMSH-LP AMSH-like protein -- AMSH-SBM SBM motif of AMSH -- CHMP charged multivesicular body proteins -- CSP chemical shift perturbation -- ESCRT endosomal sorting complexes required for transport -- Hrs hepatocyte growth factor-regulated substrate -- HSQC heteronuclear single quantum coherence -- Lys63-Ub2 Lys63-linked diubiquitin -- Lys63-Ub3 Lys63-linked triubiquitin -- MD molecular dynamics -- 15N R2 15N transverse spin relaxation rate -- NOE nuclear Overhauser enhancement -- NOESY NOE spectroscopy -- R2 spin relaxation rate -- SBM SH3 binding motif -- SH3 Src homology 3 domain -- STAM signal-transducing adaptor molecule -- TEV tobacco etch virus -- TOCSY total correlated spectroscopy -- Ub monoubiquitin -- UBD ubiquitin binding domain -- UIM ubiquitin-interacting motif -- USP ubiquitin-specific protease -- USP8 ubiquitin carboxyl-terminal hydrolase 8 -- VHS Vps27/Hrs/STAM -- JAMM Josephins, and the JAB1/MPN/MOV34 family -- UCH ubiquitin COOH-terminal hydrolyses -- OTU ovarian tumor proteases -- MJD Machado Joseph Disease -- MIT microtubule interacting and transport domain -- MIM MIT-interacting motif -- CSI Chemical Shift Index; endocytosis -- NMR -- deubiquitinating enzymes -- AMSH -- STAM2; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2016.10.002
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: AMSH [associated molecule with a Src homology 3 domain of signal transducing adaptor molecule (STAM)] is one of the deubiquitinating enzymes associated in the regulation of endocytic cargo trafficking. It shows an exquisite selectivity for Lys63-linked polyubiquitin chains that are the main chains involved in cargo sorting. The first step requires the ESCRT-0 complex that comprises the STAM and hepatocyte growth factor-regulated substrate (Hrs) proteins. Previous studies have shown that the presence of the STAM protein increases the efficiency of Lys63-linked polyubiquitin chain cleavage by AMSH, one of the deubiquitinating enzyme involved in lysosomal degradation. In the present study, we are seeking to understand if a particular structural organization among these three key players is responsible for the stimulation of the catalytic activity of AMSH. To address this question, we first monitored the interaction between the ubiquitin interacting motif (UIM)-SH3 construct of STAM2 and the Lys63-linked diubiquitin (Lys63-Ub2 ) chains by means of NMR. We show that Lys63-Ub2 is able to bind either the UIM or the SH3 domain without any selectivity. We further demonstrate that the SH3 binding motif (SBM) of AMSH (AMSH-SBM) outcompetes Lys63-Ub2 for binding SH3. Additionally, we show how different AMSH-SBM variants, modified by their sequence and length, exhibit similar equilibrium dissociation constants when binding SH3 but significantly differ in their dissociation rate constants. Finally, we report the solution NMR structure of the AMSH-SBM/SH3 complex and propose a structural organization where the AMSH-SBM interacts with the STAM2-SH3 domain and contributes to the correct positioning of AMSH prior to polyubiquitin chains' cleavage. Graphical Abstract: Highlights: The deubiquitinating enzyme AMSH specifically cleaves Lys63-linked polyubiquitin chains and increases its efficiency with the presence of the STAM protein. Both the UIM and the SH3 domain of the STAM2 protein interact with Lys63-Ub2 . The SBM of AMSH outcompetes Lys63-Ub2 for SH3 binding. The SBM is unstructured in the free state and in complex with SH3. Overall, our findings and especially the AMSH-SBM/STAM2-SH3 structure reveal a possible structural organization and mechanism among AMSH, STAM, and Lys63-Ub2 chains.