Inside Cover: A High‐Throughput Fluorescence Assay to Determine the Activity of Tryptophan Halogenases (Angew. Chem. Int. Ed. 45/2016). Issue 45 (26th September 2016)

• Record Type: Journal Article
• Authors: Schnepel, Christian; Minges, Hannah; Frese, Marcel; Sewald, Norbert
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 45(2016)
• Page Start: 13902
• Page End: 13902
• Publication Date: 2016-09-26
• Subjects: cross-coupling -- directed evolution -- halogenases -- high-throughput screening -- one-pot processes; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201609001
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : When engineering halogenases, the identification of desired mutants from a vast library is comparable to finding a needle in a haystack. In their Communication onpage 14159 ff., N. Sewald et al. highlight the application of a palladium‐catalyzed cross‐coupling to monitor halogenase activity in a high‐throughput screening. By making use of fluorogenic cross‐coupling and the formation of a fluorescent aryltryptophan, they were finally able to retrieve a thermostable tryptophan 6‐halogenase.