High‐resolution melting analysis for the detection of two erythromycin‐resistant Bordetella pertussis strains carried by healthy schoolchildren in China. (11th March 2013)

• Record Type: Journal Article
• Authors: Zhang, Q.; Li, M.; Wang, L.; Xin, T.; He, Q.
• Editors: Raoult, D.
• Is Part Of: Clinical microbiology and infection. Volume 19:Number 6(2013:Jun.)
• Page Start: E260
• Page End: E262
• Publication Date: 2013-03-11
• Subjects: Bordetella pertussis -- China -- erythromycin‐resistant -- high‐resolution melting analysis; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1111/1469-0691.12161
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Two erythromycin‐resistant strains of Bordetella pertussis were isolated from nasopharyngeal specimens of two asymptomatic schoolchildren in China. High‐resolution melting and sequencing analyses confirmed the homogeneous A2047G mutation in 23S rRNA genes of the two isolates. High‐resolution melting (HRM) analysis is a useful assay for the rapid detection of erythromycin‐resistant B. pertussis . The appearance of erythromycin‐resistant B. pertussis strains in China is alarming.