Nitric oxide protects endothelium from cadmium mediated leakiness. (7th March 2013)

• Record Type: Journal Article
• Authors: Nagarajan, Shunmugam; Rajendran, Saranya; Saran, Uttara; Priya, M. Krishna; Swaminathan, Akila; Siamwala, Jamila H.; Sinha, Swaraj; Veeriah, Vimal; Sonar, Punam; Jadhav, Vivek; Jaffar Ali, B.M.; Chatterjee, Suvro
• Is Part Of: Cell biology international. Volume 37:Number 5(2013)
• Page Start: 495
• Page End: 506
• Publication Date: 2013-03-07
• Subjects: cadmium -- cytoskeleton -- endothelial cells -- nitric oxide -- nitric oxide donor; Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10070
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Cadmium targets the vascular endothelium causing endothelial dysfunction and leakiness of endothelial barrier. Nitric oxide plays a major role in mediating endothelial functions including angiogenesis, migration and permeability. The present study investigates the nitric oxide effects on cadmium induced endothelial leakiness. Results of ex vivo and in vitro permeability assays showed that even a sub‐lethal dose of cadmium chloride (1 µM) was sufficient to induce leakiness of endothelial cells. Cadmium drastically altered the actin polymerisation pattern and membrane tension of these cells compared to controls. Addition of nitric oxide donor Spermine NONOate (SP) significantly blunted cadmium‐mediated effects and recover endothelial cells integrity. Cadmium‐induced cytoskeletal rearrangements and membrane leakiness are associated with the low nitric oxide availability and high reactive oxygen species generation. In brief, we show the protective role of nitric oxide against cadmium‐mediated endothelial leakiness.