Analysis of nasal potential in murine cystic fibrosis models. (November 2016)

• Record Type: Journal Article
• Authors: da Cunha, Mélanie Faria; Simonin, Juliette; Sassi, Ali; Freund, Romain; Hatton, Aurélie; Cottart, Charles-Henry; Elganfoud, Nadia; Zoubairi, Rachid; Dragu, Corina; Jais, Jean Philippe; Hinzpeter, Alexandre; Edelman, Aleksander; Sermet-Gaudelus, Isabelle
• Is Part Of: International journal of biochemistry & cell biology. Volume 80(2016:Nov.)
• Page Start: 87
• Page End: 97
• Publication Date: 2016-11
• Subjects: ABCC7 ATP binding cassette subfamily C member 7 -- ATP Adenosine triphosphate -- BALF Bronchoalveolar lavage fluid -- CaCC Ca2+ activated chloride channel -- cAMP 3′, 5′-cyclic adenosine monophosphate -- CF Cystic fibrosis -- CFTR Cystic fibrosis transmembrane conductance regulator -- ClC Chloride channel family -- CLCN2 Chloride channel protein 2 -- CNG Cyclic nucleotide gated -- DIDS Disodium 4, 4′-diisothiocyanatostilbene-2, 2′-disul​fonate -- DPC Diphenylamine-2-carboxylic acid -- ENaC Epithelial sodium channel -- FDA Food and Drug Administration -- FVB/NJ Friend Virus B NIH Jackson -- GlyH-101 N-(2-Naphthalenyl)-((3, 5-dibromo-2, 4-dihydroxyphenyl)methylene)glycine hydrazide -- HE Haematoxylin-eosin -- CFTR(inh)-172 5-[(4-Carboxyphenyl)methylene]-2-thioxo-3-[(3-trifluoromethyl)phenyl-4-thiazolidinone -- IP3 Inositol 1, 4, 5-trisphosphate -- Iαl Inter-α-inhabitor -- KO Knock-out -- LPC Lysophosphatidylcholine -- NPD Nasal transepithelial potential difference in humans -- OE Olfactory epithelium -- ORN Olfactory receptor neurons -- PKA Protein kinase A -- RE Respiratory epithelium -- RNA Ribonucleic acid -- Rp-cAMPS Rp-Cyclic 3′, 5′-hydrogen phosphorothioate adenosine triethylammonium salt -- TEPD Nasal transepithelial potential difference in mice -- UTP Uridine-5′-triphosphate -- VTE Transepithelial voltage -- WT Wild type; Mouse -- Cystic fibrosis -- Nasal potential difference -- CFTR correctors -- Ionic transepithelial transport; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2016.10.001
• Languages: English
• ISSNs: 1357-2725

Abstract

Abstract: The nasal epithelium of the mouse closely mimics the bioelectrical phenotype of the human airways. Ion transport across the nasal epithelium induces a nasal transepithelial potential difference. Its measurement by a relatively non-invasive method adapted from humans allows in vivo longitudinal measurements of CFTR-dependent ionic transport in the murine nasal mucosa. This test offers a useful tool to assess CFTR function in preclinical studies for novel therapeutics modulating CFTR activity. Here we extensively review work done to assess transepithelial transport in the murine respiratory epithelium in the basal state and after administration of CFTR modulators. Factors of variability and discriminative threshold between the CF and the WT mice for different readouts are discussed.