Delayed gastric emptying in diabetic rats caused by decreased expression of cystathionine gamma lyase and H2S synthesis: in vitro and in vivo studies. Issue 11 (20th June 2016)

• Record Type: Journal Article
• Authors: Mard, S. A.; Ahmadi, I.; Ahangarpour, A.; Gharib‐Naseri, M. K.; Badavi, M.
• Is Part Of: Neurogastroenterology & motility. Volume 28:Issue 11(2016)
• Page Start: 1677
• Page End: 1689
• Publication Date: 2016-06-20
• Subjects: diabetic -- gastric emptying rate -- gastroparesis -- H2S -- rat; Gastrointestinal system -- Motility -- Periodicals; Gastrointestinal system -- Innervation -- Periodicals; 616.33
• DOI: 10.1111/nmo.12867
• Languages: English
• ISSNs: 1350-1925

Abstract

Abstract: Background: This study aimed to evaluate the role of H2 S on gastric emptying rate (GER) and also to determine the effect of gastric distention on mRNA and protein expression of cystathionine β‐lyase (CBS) and cystathionine γ‐synthase (CSE) in diabetic‐gastroparetic and normal rats. Methods: Adult normal rats intraperitoneally received either propargylglycine (PAG), L‐cysteine or NaHS 30 min prior to GER marker (acetaminophen) to investigate H2 S involvement in GER and the same protocols were performed in diabetes‐induced gastroparesis rats. The role of calcitonin gene related peptide (CGRP) neurons in the prokinetic effect of endogenous H2 S on GER was determined. The level of CBS and CSE expressions in response to gastric distention were also determined. The effect of H2 S on frequency and tension of spontaneous contractions of gastric smooth muscle strips was investigated. Key Results: Our results showed that: (i) H2 S and L‐cysteine increased GER in gastroparetic and normal rats. (ii) The increased levels of CSE expression in response to gastric distention in diabetic rats were lower than in normal rats. (iii) PAG inhibited the excitatory effect of capsaicin on GER and on tension of spontaneous contractions of strips. (iv) Hydrogen sulphide increased the frequency and tension of spontaneous contractions of gastric strip muscles in normal and diabetic rats. Conclusions & Inferences: The results showed that delayed GER in diabetic rats can be due to down‐regulation of H2 S biosynthesis enzyme, CSE and suggested that a potential prokinetic role for H2 S to treat the delayed gastric emptying in diabetic patients. Abstract : The results of the present study showed that endogenous and exogenous H2 S and L‐cysteine accelerate the GER in normal and diabetic rats. The molecular findings were also showed that mRNA and protein expression of cystathionine gamma lyase, the main responsible enzyme for H2 S synthesis in the rat stomach decreased in diabetic rats.