Horseradish peroxidase-assisted approach to decolorize and detoxify dye pollutants in a packed bed bioreactor. (1st December 2016)

• Record Type: Journal Article
• Authors: Bilal, Muhammad; Iqbal, Hafiz M.N.; Hussain Shah, Syed Zakir; Hu, Hongbo; Wang, Wei; Zhang, Xuehong
• Is Part Of: Journal of environmental management. Volume 183:Part 3(2016)
• Page Start: 836
• Page End: 842
• Publication Date: 2016-12-01
• Subjects: Horseradish peroxidase -- Covalent immobilization -- Sodium-alginate matrix -- Thermo-stability -- Biodegradation -- Cytotoxicity; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2016.09.040
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: In this study, horseradish peroxidase (HRP) was covalently immobilized on the calcium-alginate support using glutaraldehyde (GA) as a cross-linking reagent for detoxification and degradation of synthetic dyes. Immobilization procedure furnished significant immobilization efficiency (86.27 ± 3.43%) along with apparent and relative activity of 24.39 ± 1.03 U/g and 84.97 ± 3.54%, respectively, for immobilized-HRP. In comparison to free-state, immobilized-HRP catalyzed the substrate oxidation reaction in a slightly acidic and wider temperature range, with an optimum at 60 °C. After 10 and 60 min of incubation at 60 °C, the immobilized-HRP displayed 99.0% and 89.0% of residual activities, whereas the free counterpart retained only 34.0% and 18.0% of residual activities, respectively. Moreover, the immobilized-HRP showed potential efficiency for the decolorization of dyes in sequential dye-decolorizing batch reactions. Cytotoxicity analysis using a plant bioassay and acute test demonstrated that the Ca-alginate immobilized-HRP may effectively be used for detoxification of dyes and has a great potential for large-scale environmental remediation. Highlights: A novel type of horseradish peroxidase (HRP) was isolated from horseradish roots. The enhanced biocatalytic potential of immobilized-HRP was recorded. Maximal dye degradation and detoxification were recorded.