AUTS2 in the nucleus accumbens is essential for heroin-induced behavioral sensitization. (1st October 2016)

• Record Type: Journal Article
• Authors: Zhu, Yongsheng; Xing, Bo; Dang, Wei; Ji, Yuanyuan; Yan, Peng; Li, Yunxiao; Qiao, Xiaomeng; Lai, Jianghua
• Is Part Of: Neuroscience. Volume 333(2016)
• Page Start: 35
• Page End: 43
• Publication Date: 2016-10-01
• Subjects: AUTS2 autism susceptibility candidate 2 -- CPu caudate-putamen -- NAc nucleus accumbens -- PcG polycomb group -- PRC polycomb repressive complex; autism susceptibility candidate 2 -- nucleus accumbens -- behavioral sensitization; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.07.007
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Decreased AUTS2 in NAc was associated with chronic heroin exposure. Heroin sensitization is correlated with lower AUTS2 expression in NAc. Knockdown AUTS2 in NAc potentiates heroin sensitization. AUTS2 overexpression in NAc elevates Rac1 activation. AUTS2 overexpression in NAc suppresses heroin sensitization. Abstract: Autism susceptibility candidate 2 (AUTS2) is a gene associated with autism and mental retardation. Recent studies have suggested an association of the AUTS2 gene with heroin dependence, and reduced AUTS2 gene expression may confer increased susceptibility to heroin dependence. However, the functional role of the AUTS2 protein in regulating enduring neuroadaptations in response to heroin exposure has not been established. Here, we investigated the effects of acute and chronic heroin exposure on AUTS2 mRNA and protein expression in the nucleus accumbens (NAc) and caudate-putamen (CPu) to determine whether changes in AUTS2 expression are associated with heroin-induced locomotor sensitization in mice. Moreover, we explored whether AUST2 knockdown affects heroin-induced locomotor sensitization. AUTS2 mRNA and protein expression in the NAc, but not the CPu, was decreased after chronic heroin (1 mg/kg) administration. In the NAc, the expression of heroin-induced locomotor sensitization was enhanced through the lentiviral-AUTS2-shRNA-mediated knockdown of AUTS2, while the overexpression of AUTS2 attenuated the locomotor-stimulant effects of heroin. Together, these results indicate that AUTS2 in the NAc, but not the CPu, suppresses the initiation and expression of heroin-induced behavioral sensitization, suggesting that AUST2 may be a potential target for the treatment of heroin dependence.