Genetic variability of the phloem sap metabolite content of maize (Zea mays L.) during the kernel-filling period. (November 2016)

• Record Type: Journal Article
• Authors: Yesbergenova-Cuny, Zhazira; Dinant, Sylvie; Martin-Magniette, Marie-Laure; Quilleré, Isabelle; Armengaud, Patrick; Monfalet, Priscilla; Lea, Peter J.; Hirel, Bertrand
• Is Part Of: Plant science. Volume 252(2016:Nov.)
• Page Start: 347
• Page End: 357
• Publication Date: 2016-11
• Subjects: ADMIS Automated spectral deconfolution and identification system -- C carbon -- CC companion cells -- FW fresh weight -- GC gas chromatography -- GS glutamine synthetase -- HCA hierarchical clustering analysis -- KN kernel number -- KY kernel yield -- LOWESS locally weighted scatterplot smoothing -- MS mass spectrometry -- N nitrogen -- NetCDF network common data form -- STE sieve tube elements -- TKW thousand kernel weight; Exudates -- Genetic variability -- Maize -- Metabolome -- Phloem -- Metabolism -- Yield; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2016.08.007
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Variability of the phloem sap metabolite composition was observed in seventeen maize lines representative of maize genetic diversity. No obvious relationship between the phloem sap composition and the geographical origin of the different lines was found. Correlation studies indicated that a number of phloem sap carbohydrates could act as good metabolic markers of kernel yield. Abstract: Using a metabolomic approach, we have quantified the metabolite composition of the phloem sap exudate of seventeen European and American lines of maize that had been previously classified into five main groups on the basis of molecular marker polymorphisms. In addition to sucrose, glutamate and aspartate, which are abundant in the phloem sap of many plant species, large quantities of aconitate and alanine were also found in the phloem sap exudates of maize. Genetic variability of the phloem sap composition was observed in the different maize lines, although there was no obvious relationship between the phloem sap composition and the five previously classified groups. However, following hierarchical clustering analysis there was a clear relationship between two of the subclusters of lines defined on the basis of the composition of the phloem sap exudate and the earliness of silking date. A comparison between the metabolite contents of the ear leaves and the phloem sap exudates of each genotype, revealed that the relative content of most of the carbon- and nitrogen-containing metabolites was similar. Correlation studies performed between the metabolite content of the phloem sap exudates and yield-related traits also revealed that for some carbohydrates such as arabitol and sucrose there was a negative or positive correlation with kernel yield and kernel weight respectively. A posititive correlation was also found between kernel number and soluble histidine.