Effects of geographic area, feedstock, temperature, and operating time on microbial communities of six full-scale biogas plants. (October 2016)

• Record Type: Journal Article
• Authors: Fontana, Alessandra; Patrone, Vania; Puglisi, Edoardo; Morelli, Lorenzo; Bassi, Daniela; Garuti, Mirco; Rossi, Lorella; Cappa, Fabrizio
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 980
• Page End: 990
• Publication Date: 2016-10
• Subjects: Illumina sequencing -- Metagenomics -- Microbiome -- 16S rRNA gene -- Anaerobic digestion; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.07.058
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Investigation on microbiomes of Parmigiano Reggiano and Grana Padano biogas plants. Methanosarcina abundance correlates with ammonium concentration. Methanoculleus more present under thermophilic conditions. Thermotogales correlates with hydraulic retention time. Acetate levels seems to influence Methanosarcina and Methanosaeta distribution. Abstract: The objective of this study was to investigate the effect of different animal feedings operated in two distinct PDO (protected designation of origin) cheese production areas (Parmigiano Reggiano and Grana Padano) on the microbiome of six full-scale biogas plants, by means of Illumina sequencing and qPCR techniques. The effects of feedstock (cattle slurry manure, energy crops, agro-industrial by-products), temperature (mesophilic/thermophilic), and operating time were also examined, as were the relationships between the predominant bacterial and archaeal taxa and process parameters. The different feedstocks and temperatures strongly affected the microbiomes. A more biodiverse archaeal population was highlighted in Parmigiano Reggiano area plants, suggesting an influence of the different animal feedings. Methanosarcina and Methanosaeta showed an opposite distribution among anaerobic plants, with the former found to be related to ammonium concentration. The Methanoculleus genus was more abundant in the thermophilic digester whereas representation of the Thermotogales order correlated with hydraulic retention time.