Enhanced mannan-derived fermentable sugars of palm kernel cake by mannanase-catalyzed hydrolysis for production of biobutanol. (October 2016)

• Record Type: Journal Article
• Authors: Shukor, Hafiza; Abdeshahian, Peyman; Al-Shorgani, Najeeb Kaid Nasser; Hamid, Aidil Abdul; Rahman, Norliza A.; Kalil, Mohd Sahaid
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 257
• Page End: 264
• Publication Date: 2016-10
• Subjects: Palm kernel cake -- Enzymatic hydrolysis -- Mannanase -- Mannan-derived monomeric sugar -- Biobutanol production; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.06.084
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Palm kernel cake (PKC) was hydrolyzed by mannanase. The effect of operating factors on the enzymatic hydrolysis of PKC was studied. Mannose was the main fermentable sugar released with glucose to a lower extent. The total sugar of 71.54 ± 2.54 g/L was produced in optimum conditions. Maximum biobutanol production of 3.27 ± 1.003 g/L was obtained. Abstract: Catalytic depolymerization of mannan composition of palm kernel cake (PKC) by mannanase was optimized to enhance the release of mannan-derived monomeric sugars for further application in acetone-butanol-ethanol (ABE) fermentation. Efficiency of enzymatic hydrolysis of PKC was studied by evaluating effects of PKC concentration, mannanase loading, hydrolysis pH value, reaction temperature and hydrolysis time on production of fermentable sugars using one-way analysis of variance (ANOVA). The ANOVA results revealed that all factors studied had highly significant effects on total sugar liberated (P < 0.01). The optimum conditions for PKC hydrolysis were 20% (w/v) PKC concentration, 5% (w/w) mannanase loading, hydrolysis pH 4.5, 45 °C temperature and 72 h hydrolysis time. Enzymatic experiments in optimum conditions revealed total fermentable sugars of 71.54 ± 2.54 g/L were produced including 67.47 ± 2.51 g/L mannose and 2.94 ± 0.03 g/L glucose. ABE fermentation of sugar hydrolysate by Clostridium saccharoperbutylacetonicum N1-4 resulted in 3.27 ± 1.003 g/L biobutanol.