Effect of moderate static electric field on the growth and metabolism of Chlorella vulgaris. (October 2016)

• Record Type: Journal Article
• Authors: Nezammahalleh, Hassan; Ghanati, Faezeh; Adams, Thomas A.; Nosrati, Mohsen; Shojaosadati, Seyed Abbas
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 700
• Page End: 711
• Publication Date: 2016-10
• Subjects: Cell metabolism -- Chlorella vulgaris -- Electroporation -- Growth stimulation -- Inoculum size -- Peroxide accumulation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.07.018
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Interaction of electric field (EF) with Chlorella vulgaris was studied. C. vulgaris showed a hormetic response to pretreatment with moderate intensity EF. A 50 min pretreatment of 0.4 g L −1 algal suspension lead to 51% growth stimulation. The growth stimulation of C. vulgaris is highly affected by the algal concentration. The inoculum size strongly affects both growth and metabolism of C. vulgaris. Abstract: An electric field (EF) generator device was fabricated and applied to the treatment of Chlorella vulgaris ISC33 at three distinct concentrations before cultivation. The EF of moderate intensity (2.7 kV cm −1 ) has a hormetic effect on algal growth. The highest growth stimulation of 51% was observed after 50 min treatment of 0.4 g L −1 algal suspension. The influence of EF on the system was then studied from both theoretical and experimental perspectives. The growth rate increased with treatment time up to a maximum because of improved membrane permeability, and then declined afterwards due to peroxide accumulation in the medium. The contents of chlorophylls, carotenoids, soluble carbohydrates, lipids, and proteins were also measured to understand possible changes on algal metabolism. The EF treatment of algal suspension has no observable effect on the cell metabolism while both algal growth and metabolism was significantly affected by the inoculum size.