In situ detoxification of dry dilute acid pretreated corn stover by co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae increases ethanol production. (October 2016)

• Record Type: Journal Article
• Authors: Zhu, Jia-Qing; Li, Xia; Qin, Lei; Li, Wen-Chao; Li, Hui-Ze; Li, Bing-Zhi; Yuan, Ying-Jin
• Is Part Of: Bioresource technology. Volume 218(2016)
• Page Start: 380
• Page End: 387
• Publication Date: 2016-10
• Subjects: Co-culture fermentation -- Simultaneous saccharification and co-fermentation -- Saccharomyces cerevisiae -- Inhibitor tolerant -- Xylose-utilizing -- High content inhibitors; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.06.107
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A novel co-culture system was developed for bioethanol production. Glucose accumulation in fermentation using XS was eliminated by introduction of TS. Co-culture fermentation improved xylose utilization and ethanol production. Co-culture SSCF increased ethanol concentration by 21.2% and 41.0%. Undetoxified corn stover from dry dilute acid pretreatment was bio-converted. Abstract: Co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae was developed for bioethanol production from undetoxified pretreated biomass in simultaneously saccharification and co-fermentation (SSCF) process. Glucose accumulation during late fermentation phase in SSCF using xylose-utilizing strain can be eliminated by the introduction of inhibitor-tolerant strain. Effect of different ratios of two strains was investigated and xylose-utilizing strain to inhibitor-tolerant strain ratio of 10:1 (w/w) showed the best xylose consumption and the highest ethanol yield. Inoculating of xylose-utilizing strain at the later stage of SSCF (24–48 h) exhibited lower ethanol yield than inoculating at early stage (the beginning 0–12 h), probably due to the reduced enzymatic efficiency caused by the unconsumed xylose and oligomeric sugars. Co-culture SSCF increased ethanol concentration by 21.2% and 41.0% comparing to SSCF using individual inhibitor-tolerant and xylose-utilizing strain (increased from 48.5 and 41.7 g/L to 58.8 g/L), respectively, which suggest this co-culture system was very promising.