Effects of commonly used intravitreal anti‐vascular endothelial growth factor drugs on mesenchymal stem cells derived from the limbus and ciliary body. (19th March 2016)

• Record Type: Journal Article
• Authors: Acar, Ugur; Erginturk Acar, Damla; Alpaslan Pinarli, Ferda; Demir, Muhammed N; Beyazyildiz, Emrullah; Ozdemir, Ozdemir; Gulmez Sevim, Duygu; Tiryaki, Meral; Sobaci, Gungor
• Is Part Of: Clinical & experimental ophthalmology. Volume 44:Number 7(2016)
• Page Start: 587
• Page End: 596
• Publication Date: 2016-03-19
• Subjects: Aflibercept -- anti‐VEGF -- bevacizumab -- ciliary body -- intravitreal injection -- limbus -- mesencyhmal stem cell -- ranibizumab; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1111/ceo.12715
• Languages: English
• ISSNs: 1442-6404

Abstract

Abstract: Background: To investigate the effects of commonly used intravitreal anti‐vascular endothelial growth factor (anti‐VEGF) antibodies on proliferation index and viability of mesenchymal stem cells derived from ciliary body and limbus (CB‐MSC and LMSC). Methods: CB‐MSCs and LMSCs were isolated from newborn rats' eyes, and they were expanded in medium by the explant method. Intravitreally used anti‐VEGF drugs, aflibercept, bevacizumab and ranibizumab were tested into the 16‐well plates, respectively, at four different concentrations. After keeping them for 48 h, the proliferation indexes and viabilities of CB‐MSCs and LMSCs were compared separately by Real‐Time Cell Analyzer and Methylthiazoltetrazoli (MTT) test. Results: Anti‐VEGFs used at 5‐times and 10‐times of the standard clinical dosage caused statistically significant negative effects on proliferation indexes of CB‐MSCs and LMSCs at the 24 th hour compared to control group. Only the anti‐VEGF group that had 10‐times dosage of those used clinically had a statistically significant negative effect on the viabilties of CB‐MSCs and LMSCs. Conclusion: Administrations of high doses or repeated standard doses of intravitreal anti‐VEGF agents may affect the proliferation indexes and viabilities of CB‐MSCs and LMSCs adversely. These novel findings deserve further in vivo investigations.