Comparative analysis of micro‐RNAs in human papillomavirus–positive versus –negative oropharyngeal cancers. Issue 11 (21st April 2016)

• Record Type: Journal Article
• Authors: Mirghani, Haitham; Ugolin, Nicolas; Ory, Catherine; Goislard, Maud; Lefèvre, Marine; Baulande, Sylvain; Hofman, Paul; Guily, Jean Lacau St; Chevillard, Sylvie; Lacave, Roger
• Is Part Of: Head & neck. Volume 38:Issue 11(2016)
• Page Start: 1634
• Page End: 1642
• Publication Date: 2016-04-21
• Subjects: oropharyngeal/oropharynx cancer -- human papillomavirus 16 -- microarray -- micro/miRNA -- gene expression profile -- PI3K pathway -- Wnt pathway; Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.24487
• Languages: English
• ISSNs: 1043-3074

Abstract

Abstract: Background: Oncogenic mechanisms of human papillomavirus (HPV)‐positive oropharyngeal cancer are still poorly characterized. Analysis of their microRNA expression profile might provide valuable information. Methods: The microRNA expression profiles were analyzed by micro‐arrays in 26 oropharyngeal cancers. A microRNA signature specific to HPV‐status was identified by analyzing a learning/training set consisting of 16 oropharyngeal cancers. The robustness of this signature was further confirmed by blind case‐by‐case classification of a validation set composed of 10 independent tumors. Putative targeted molecular pathways were proposed using DIANA miRPath online software (http://microrna.gr/mirpath) . Results: We have identified 25 miRNA signatures, which discriminates HPV16‐positive oropharyngeal cancer from their HPV‐negative counterparts. These 25 microRNAs play a potential role in Wnt and PI3K‐pathways, cell‐adhesion/cell‐polarity, and the cytoskeleton regulation. Conclusion: Our study contributes to a better understanding of pathogenic mechanisms involved in the development of HPV‐positive oropharyngeal cancer and in the identification of potential therapeutic molecular targets. © 2016 Wiley Periodicals, Inc. Head Neck 38 : 1708–1716, 2016