The c.–360 T > C mutation affects PGAM2 transcription activity and is linked with the water holding capacity of the longissimus lumborum muscle in pigs. (December 2016)

• Record Type: Journal Article
• Authors: Yang, Haoxin; He, Jiawen; Wei, Wei; Chu, Weiwei; Yu, Shigang; Tian, Ye; Peng, Fengyi; Liu, Hongcheng; Zhang, Zengkai; Chen, Jie
• Is Part Of: Meat science. Volume 122(2016:Dec.)
• Page Start: 139
• Page End: 144
• Publication Date: 2016-12
• Subjects: Water holding capacity -- PGAM2 -- Mutation -- Pork; Meat -- Periodicals; Meat industry and trade -- Periodicals; Viande -- Périodiques; Viande -- Industrie -- Périodiques; Meat; Meat industry and trade; Periodicals; 641.36
• DOI: 10.1016/j.meatsci.2016.07.023
• Languages: English
• ISSNs: 0309-1740

Abstract

Abstract: The phosphoglycerate mutase 2 ( PGAM2 ) gene encodes a key enzyme in the glycolytic process. This study examined a functional mutation in the PGAM2 gene and evaluated its relationship with water holding capacity (WHC). RT-qPCR analysis showed the PGAM2 mRNA level was significantly higher in the low-WHC group than in the high-WHC group ( P < 0.05). The c.–360 T > C mutation was identified through sequencing and found to have opposite allele distributions in the two groups. The allele was further genotyped in 170 Duroc × Large White × Yorkshire crossbred pigs using allele-specific PCR. The CC genotype was associated with lower WHC and higher PGAM2 mRNA levels, whereas the TT genotype corresponded to a higher WHC and lower PGAM2 mRNA levels ( P < 0.05). A luciferase activity assay also showed that the CC-genotype promoter had higher activity than the TT-genotype promoter ( P < 0.05). In conclusion, we discovered the c.–360 T > C mutation in the PGAM2 gene, which is a promising marker for improving pork WHC. Highlights: One SNP was found in the promoter of PGAM2 gene. PGAM2 gene has an association with water holding capacity. TT genotype could be a promising genetic markers for the selection of pork with high water holding capacity.