Androgen induces gonadal soma-derived factor, Gsdf, in XX gonads correlated to sex-reversal but not Dmrt1 directly, in the teleost fish, northern medaka (Oryzias sakaizumii). (15th November 2016)

• Record Type: Journal Article
• Authors: Horie, Yoshifumi; Myosho, Taijun; Sato, Tadashi; Sakaizumi, Mitsuru; Hamaguchi, Satoshi; Kobayashi, Tohru
• Is Part Of: Molecular and cellular endocrinology. Volume 436(2016)
• Page Start: 141
• Page End: 149
• Publication Date: 2016-11-15
• Subjects: Gsdf -- XX sex-reversal -- Androgen -- Medaka -- Inbred HNI-II strain -- Oryzias sakaizumii; Gsdf gonadal soma-derived factor -- Dmy DM domain gene on Y chromosome -- Dmrt1 doublesex/mab-3 related transcription factor-1 -- dpf days post fertilization -- dph days post hatching -- q-PCR quantitative RT-PCR -- ISH in situ hybridization -- MT 17α-methyltestosterone; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2016.07.022
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: In the inbred HNI-II strain of Oryzias sakaizumii, Dmy and Gsdf are expressed in XY gonads from Stages 35 and 36, respectively, similarly to the inbred Hd-rR strain of Oryzias latipes . However, Dmrt1 respectively becomes detectable at Stage 36 and 5 days post hatching (dph) in the two strains. In XX HNI-II embryos, 17α-methyltestosterone (MT) induces Gsdf mRNA from Stage 36, accompanied by complete sex-reversal in all treated individuals (MT, 10 ng/mL), while Dmrt1 mRNA was first detectable at 5 dph. In XX d-rR, MT induced Gsdf mRNA expression and sex-reversal in only some of the treated individuals. Together, these results suggest the testis differentiation cascade in XY individuals differs between the HNI-II and Hd-rR strains. In addition, it is suggested that androgen-induced XX sex-reversal proceeds via an androgen- Gsdf - Dmrt1 cascade and that Gsdf plays an important role in sex-reversal in medaka. Highlights: In XY Oryzias sakaizumii, the testis determination pathway differs from Oryzias latipes . Testis determination proceeds in parallel via both Dmy-Gsdf-Dmrt1 and Dmy-Dmrt1 . In XX embryos, MT induces Gsdf, but not Dmrt1 directly. Gsdf plays an important role in androgen-induced XX sex-reversal in medaka.