P7. Effect on cell cycle proliferation rates of cell lines using gold nanoparticles. (September 2016)

• Record Type: Journal Article
• Authors: Muller, X.; Engelbrecht, M.; Slabbert, J.P.
• Is Part Of: Physica medica. Volume 32(2016)Supplement 2
• Page Start: 163
• Publication Date: 2016-09
• Subjects: Medical physics -- Periodicals; Biophysics -- Periodicals; Biophysics -- Periodicals; Imagerie médicale -- Périodiques; Radiothérapie -- Périodiques; Rayons X -- Sécurité -- Mesures -- Périodiques; Physique -- Périodiques; Médecine -- Périodiques; 610.153
• DOI: 10.1016/j.ejmp.2016.07.074
• Languages: English
• ISSNs: 1120-1797

Abstract

Abstract : Introduction: As nanoparticles have vast multifunctionalities, a great interest in its use in cancer therapy applications has been on the rise. Gold nanoparticles (AuNPs) in cancer therapy have shown promising results due to their cytotoxic abilities. In this study, the radiosensitizing effect of green synthesized AuNPs was compared with the well-known chemically synthesized Turkevich method. This was done by assessing their effect on the cell cycle proliferation rate of 3 different cell lines with the aid of the thymidine analogue bromodeoxyuridine (BrdU). Materials and methods: AuNPs was synthesized via the green chemistry and Turkevich methods. CHO-K1, BEnd5 and HaCaT cell lines were incubated with 5 μg/ml of both AuNP types for 4 h and overnight. A BrdU pulse labelling was done 1 h prior to stopping the cultures, followed by counterstaining using a BrdU antibody and staining kit. Analysis was done using flow cytometry. Results: An average increased delay in both the S-phase and G2 phases for the AuNPs containing samples was observed. This was viewed for both the 4 h as well as overnight incubated samples. An average increase of 10–20% in the lagging of the S and G2 phases was noted for the Turkevich AuNPs in comparison with the green synthesized AuNPs for all the cell lines. Conclusion: An increase in the S and G2 phase indicates increased DNA damage caused by the AuNPs due to their cytotoxicity. A possible reason for the Turkivich AuNPs being more toxic than the green synthesized AuNPs is thought to be due to the latter containing a reservoir of phytochemicals causing a cytoprotective effect. The samples incubated with AuNPs overnight displayed a lower yield of cells which could be indicative of possible apoptosis.