O42. Realizing the European Network of Biological Dosimetry 'RENEB': Results of 2 intercomparison exercises for the micronucleus assay. (September 2016)

• Record Type: Journal Article
• Authors: Depuydt, J.; Baeyens, A.; Barnard, S.; Beinke, C.; Benedek, A.; Beukes, P.; Buraczewska, I.; Darroudi, F.; De Sanctis, S.; Dominguez, I.; Monteiro Gil, O.; Hadjidekova, V.; Kis, E.; Kulka, U.; Lista, F.; Lumniczky, K.; M'kacher, R.; Moquet, J.; Obreja, D.; Oestreicher, U.; Pajic, J.; Pastor, N.; Popova, L.; Regalbuto, E.; Ricoul, M.; Sabatier, L.; Slabbert, J.P.; Sommer, S.; Testa, A.; Thierens, H.; Wojcik, A.; Vral, A.;
• Is Part Of: Physica medica. Volume 32(2016)Supplement 2
• Page Start: 154
• Page End: 155
• Publication Date: 2016-09
• Subjects: Micronucleus assay -- Biological dosimetry -- RENEB -- Radiation protection -- Triage -- Large-scale radiation accident; Medical physics -- Periodicals; Biophysics -- Periodicals; Biophysics -- Periodicals; Imagerie médicale -- Périodiques; Radiothérapie -- Périodiques; Rayons X -- Sécurité -- Mesures -- Périodiques; Physique -- Périodiques; Médecine -- Périodiques; 610.153
• DOI: 10.1016/j.ejmp.2016.07.050
• Languages: English
• ISSNs: 1120-1797

Abstract

Abstract : Introduction: In the framework of the RENEB project, two intercomparison exercises were conducted to assess the suitability of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual scoring procedures. Results: The dose estimates provided by the network laboratories were in good agreement with the true administered doses. The most accurate estimates were reported for the low dose points ( < 1 Gy). For higher dose points ( > 2.5 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two intercomparsion exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories. The results further emphasize the importance of performing regular intercomparison training exercises within a specialised biodosimetry network.