Anti-inflammatory and anti-biofilm properties of ent-nemorosone from Brazilian geopropolis. (October 2016)

• Record Type: Journal Article
• Authors: da Cunha, Marcos Guilherme; Franchin, Marcelo; de Paula-Eduardo, Laila Facin; Freires, Irlan Almeida; Beutler, John A.; de Alencar, Severino Matias; Ikegaki, Masaharu; Tabchoury, Cinthia Pereira Machado; Cunha, Thiago Mattar; Rosalen, Pedro Luiz
• Is Part Of: Journal of functional foods. Volume 26(2016)
• Page Start: 27
• Page End: 35
• Publication Date: 2016-10
• Subjects: Geopropolis -- ent-Nemorosone -- Anti-inflammatory -- Anti-biofilm; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.07.009
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: ent -Nemorosone had no reports about its anti-inflammatory and anti-biofilm activities. ent -Nemorosone reduced the TNF-α production by acting on ERK1/2 and NF-kB. ent -Nemorosone reduced the biofilm adherence by acting on polysaccharide formation. Abstract: ent -Nemorosone is a benzophenone isolated from geopropolis from Melipona scutellaris whose biological properties remain unexplored. Herein, we evaluated the anti-inflammatory activity of ent -nemorosone by in vivo neutrophil migration and TNF-α quantification, as well as by in vitro TNF-α quantification, ERK 1/2 phosphorylation, NF-kB activation and nuclear translocation of p65 in stimulated macrophages. ent -Nemorosone was also tested for its antimicrobial effects against Streptococcus mutans biofilm adhesion, polysaccharide production, and viability. ent -Nemorosone reduced the influx of neutrophils and TNF-α release into the peritoneal cavity of mice, as well as the in vitro TNF-α levels, ERK 1/2 phosphorylation, NF-kB activation and nuclear p65 translocation. Furthermore, this compound inhibited biofilm adhesion and reduced the amount of extra- and intracellular polysaccharides synthesized by S. mutans . Thus, ent -nemorosone showed promising anti-inflammatory activity by acting on TNF-α release in macrophages, as well as anti-biofilm properties by reducing S. mutans polysaccharide production.