Multiple small versus few large amount aspirations for bone marrow harvesting in autologous and allogeneic bone marrow transplantation. Issue 2 (October 2016)

• Record Type: Journal Article
• Authors: Witt, Volker; Pichler, Herbert; Fritsch, Gerhard; Peters, Christina
• Is Part Of: Transfusion and apheresis science. Volume 55:Issue 2(2016)
• Page Start: 221
• Page End: 224
• Publication Date: 2016-10
• Subjects: Bone marrow -- Harvesting -- HPC-BM -- Stem cell transplantation; Blood -- Transfusion -- Periodicals; Hemapheresis -- Periodicals; 615.39
• DOI: 10.1016/j.transci.2016.07.017
• Languages: English
• ISSNs: 1473-0502

Abstract

Abstract: For successful bone marrow transplantation it is necessary to obtain enough progenitor cells during the bone marrow (BM) harvesting procedure. Most centers are using multiple aspirations of maximum 2 ml BM (A), while other centers are using few larger amount aspirations for BM harvesting (B). There is still a discussion about possible differences in graft composition between A and B. To evaluate the feasibility in children we evaluated twenty BM harvestings that were performed in 18 donors, 7 autologous (median age 6.93y; 2.48–16.6) and 13 allogeneic donors (median age 19.75y; 6.45–50.7). A and B were performed crosswise by 2 operators starting with A (2 ml) or B (100 ml) changing to B or A, collecting identically amounts with both methods. We found no statistically significant difference between A and B for MNC, T-cells, and CFU (MNC/ml 824572 versus 725000, p = 0.728; MNC/kg 3.1 10 7 versus 2.9 10 7, p = 0.296; CD3/ml 162500 versus 300000, p = 0.310; CFU/10 5 MNC 1678 versus 1315, p = 0.094), but for CD34+ cells (CD34/kg 2.62 versus 2.09, p = 0.045). BM harvest by the large amount few punctures method (B) is as sufficient as the commonly used small amount frequent punctures method (A), and could be therefore used equally.