Identification and functional characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei. (April 2016)

• Record Type: Journal Article
• Authors: Chen, Yong-Gui; Yuan, Kai; Zhang, Ze-Zhi; Yuan, Feng-Hua; Weng, Shao-Ping; Yue, Hai-Tao; He, Jian-Guo; Chen, Yi-Hong
• Is Part Of: Developmental and comparative immunology. Volume 57(2016)
• Page Start: 57
• Page End: 66
• Publication Date: 2016-04
• Subjects: Toll-like recepter -- NF-κB -- Solute carrier family 15 member A 4 -- Unfolded protein response -- Litopenaeus vannamei -- Vibrio parahemolyticus; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.12.005
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Innate immunity in shrimp is important in resisting bacterial infection. The NF-κB pathway is pivotal in such an immune response. This study cloned and functionally characterized the solute carrier family ( SLC ) 15 member A 4 ( LvSLC15A4 ) gene in Litopenaeus vannamei . The open reading frame of LvSLC15A4 is 1, 902 bp long and encodes a putative 633-amino acid protein, which is localized in the plasma membrane and intracellular vesicular compartments. Results of the reporter gene assay showed that LvSLC15A4 upregulated NF-κB target genes, including the immediate-early gene 1 of white spot syndrome virus, as well as several antimicrobial peptide genes, such as pen4, CecA, AttA, and Mtk in S2 cells. Moreover, knocked-down expression of LvSLC15A4 reduced pen4 expression in L. vannamei . LvSLC15A4 down-regulation also increased the cumulative mortality of Vibrio parahemolyticus -infected L. vannamei . Furthermore, LvSLC15A4 expression was induced by unfolded protein response (UPR) in L. vannamei hematocytes. These results suggest that LvSLC15A4 participates in L. vannamei innate immunity via the NF-κB pathway and thus may be related to UPR. Highlights: Identification and characterization of a solute carrier family 15, member 4 gene in Litopenaeus vannamei ( LvSLC15A4 ). LvSLC15A4 upregulated NF-κB target genes in S2 cells. In L. vanname, knocked-down LvSLC15A4 caused a higher cumulative mortality upon Vibrio parahaemolyticus. LvSLC15A4 was induced by unfolded protein response (UPR) in hematocytes of L. vannamei.