First evidence for a Vibrio strain pathogenic to Mytilus edulis altering hemocyte immune capacities. (April 2016)

• Record Type: Journal Article
• Authors: Ben Cheikh, Yosra; Travers, Marie-Agnès; Morga, Benjamin; Godfrin, Yoann; Rioult, Damien; Le Foll, Frank
• Is Part Of: Developmental and comparative immunology. Volume 57(2016)
• Page Start: 107
• Page End: 119
• Publication Date: 2016-04
• Subjects: Innate immunity -- Molluscs -- Cell-mediated immune response -- Bacterial infections -- Green fluorescent protein; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.12.014
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Bacterial isolates were obtained from mortality events affecting Mytilus edulis and reported by professionals in 2010–2013 or from mussel microflora. Experimental infections allowed the selection of two isolates affiliated to Vibrio splendidus/Vibrio hemicentroti type strains: a virulent 10/068 1T1 (76.6% and 90% mortalities in 24 h and 96 h) and an innocuous 12/056 M24T1 (0% and 23.3% in 24 h and 96 h). These two strains were GFP-tagged and validated for their growth characteristics and virulence as genuine models for exposure. Then, host cellular immune responses to the microbial invaders were assessed. In the presence of the virulent strain, hemocyte motility was instantaneously enhanced but markedly slowed down after 2 h exposure. By contrast, hemocyte velocity increased in the presence of the innocuous 12/056 M24T1. At the same time interval, 10/068 1T1 invaded hemocytes and was more rapidly internalized than the innocuous strain. Extracellular products (ECPs) prepared from 10/068 1T1 cultures significantly inhibited phagocytic activity while 12/056 M24T1 ECPs had no effect. Furthermore, the pathogenic strain and its ECPs inhibited oxidative burst unlike 12/056 M24T1 strain/ECPs which enhanced ROS production. Taken together, our results suggest that the mussel pathogen 10/068 1T1 may escape immune response by altering hemocytes functions. Highlights: ∙ Isolation of a Vibrio strain pathogenic to the blue mussel. ∙ Two differentially virulent Vibrio strains were GFP-labeled. ∙ The virulent strain altered hemocyte motility and quenched ROS production. ∙ ECPs from the virulent strain inhibited bead phagocytosis and ROS production. ∙ The virulent strain was rapidly internalized in hemocytes.