Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells. (February 2016)

• Record Type: Journal Article
• Authors: Yamaguchi, Takuya; Miyata, Shunsuke; Katakura, Fumihiko; Nagasawa, Takahiro; Shibasaki, Yasuhiro; Yabu, Takeshi; Fischer, Uwe; Nakayasu, Chihaya; Nakanishi, Teruyuki; Moritomo, Tadaaki
• Is Part Of: Fish & shellfish immunology. Volume 49(2016:Mar.)
• Page Start: 225
• Page End: 229
• Publication Date: 2016-02
• Subjects: Fish -- Carp -- IL-4/13B -- Recombinat protein -- B cell -- Proliferation -- IL-4; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.12.043
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp ( Cyprinus carpio ) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM + B cells, because after four days of stimulation the IgM + fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM + cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B. Highlights: An IL-4/13B gene was identified in common carp ( Cyprinus carpio ). The recombinant protein of carp IL-4/13B was produced. Carp recombinant IL-4/13B induced in vitro proliferation of IgM + B cell.