Developmental validation of the PowerPlex® Fusion 6C System. (March 2016)

• Record Type: Journal Article
• Authors: Ensenberger, Martin G.; Lenz, Kristy A.; Matthies, Learden K.; Hadinoto, Gregory M.; Schienman, John E.; Przech, Angela J.; Morganti, Michael W.; Renstrom, Daniel T.; Baker, Victoria M.; Gawrys, Kori M.; Hoogendoorn, Marlijn; Steffen, Carolyn R.; Martín, Pablo; Alonso, Antonio; Olson, Hope R.; Sprecher, Cynthia J.; Storts, Douglas R.
• Is Part Of: Forensic science international. Volume 21(2016:Mar.)
• Page Start: 134
• Page End: 144
• Publication Date: 2016-03
• Subjects: PowerPlex® -- Fusion 6C -- Validation -- Short tandem repeat -- STR -- Forensic science -- DNA typing -- CODIS; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2015.12.011
• Languages: English
• ISSNs: 1872-4973

Abstract

Highlights: The PowerPlex ® Fusion 6C System is a new 6-dye, 27-locus, STR multiplex. A multisite study following SWGDAM guidelines was completed for the multiplex. Results demonstrate the robustness of design and suitability for forensic use. Abstract: The PowerPlex ® Fusion 6C System is a 27-locus, six-dye, multiplex that includes all markers in the expanded CODIS core loci and increases overlap with STR database standards throughout the world. Additionally, it contains two, rapidly mutating, Y-STRs and is capable of both casework and database workflows, including direct amplification. A multi-laboratory developmental validation study was performed on the PowerPlex ® Fusion 6C System. Here, we report the results of that study which followed SWGDAM guidelines and includes data for: species specificity, sensitivity, stability, precision, reproducibility and repeatability, case-type samples, concordance, stutter, DNA mixtures, and PCR-based procedures. Where appropriate we report data from both extracted DNA samples and direct amplification samples from various substrates and collection devices. Samples from all studies were separated on both Applied Biosystems 3500 series and 6-dye capable 3130 series Genetic Analyzers and data is reported for each. Together, the data validate the design and demonstrate the performance of the PowerPlex ® Fusion 6C System.