Development of a quantitative immuno-affinity test column assay for on-site screening of clindamycin residues in milk. (April 2016)

• Record Type: Journal Article
• Authors: Wang, Xiaomei; Luo, Pengjie; Chen, Jinlian; Huang, Yuting; Jiang, Wenxiao
• Is Part Of: International dairy journal. Volume 55(2016:Apr.)
• Page Start: 59
• Page End: 63
• Publication Date: 2016-04
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2015.12.002
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: A sensitive and specific monoclonal antibody against clindamycin (CLIN) was produced and used to develop an immuno-affinity test column (IATC) assay for on-site screening of CLIN residues in milk. The qualitative limit of detection of the IATC assay, estimated as 1.0 μg L −1 by visual detection, was sufficient to measure maximum residue levels for lincosamide antibiotics in milk. The quantitative IATC assay resulted in a lower detection limit (0.11 μg L −1 ) by evaluating the colour intensity of the test layers, which was approximately 9 times greater sensitivity than visual detection. During the spike and recovery test, the average recoveries ranged from 76% to 114% at different spiked levels, and the intra-/interday coefficients of variation were in the range 9.6–16.7%. The detection time was shortened to 20 min, whereas the sensitivity was comparable with a traditional ELISA. The IATC assay shows promise for on-site screening of CLIN residues in milk.