Design of starch functionalized biodegradable P(MAA-co-MMA) as carrier matrix for l-asparaginase immobilization. (20th November 2016)

• Record Type: Journal Article
• Authors: Ulu, Ahmet; Koytepe, Suleyman; Ates, Burhan
• Is Part Of: Carbohydrate polymers. Volume 153(2016)
• Page Start: 559
• Page End: 572
• Publication Date: 2016-11-20
• Subjects: l-Asparaginase -- Enzyme immobilization -- P(MAA-co-MMA) -- Starch -- Biocomposite; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.08.019
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: P(MAA-co-MMA)-starch composite were prepared and were used as carrier matrix for the immobilization ofl -asparaginase. Due to degradability of starch, the composite was more degradation than the copolymer. Immobilizedl -asparaginase showed stability towards temperature & pH, and storage compared to free one. Affinity between the substrate andl -asparaginase improved after immobilization. Abstract: We prepared biodegradable P(MAA-co-MMA)-starch composite as carrier matrix for the immobilization ofl -asparaginase (l -ASNase), an important chemotherapeutic agent in acute lymphoblastic leukemia. Chemical characteristics and thermal stability of the prepared composites were determined by FT-IR, TGA, DTA and, DSC, respectively. Also, biodegradability measurements of P(MAA-co-MMA)-starch composites were carried out to examine the effects of degradation of the starch. Then, l -ASNase was immobilized on the P(MAA-co-MMA)-starch composites. The surface morphology of the composite before and after immobilization was characterized by SEM, EDX, and AFM. The properties of the immobilizedl -ASNase were investigated and compared with the free enzyme. The immobilizedl -ASNase had better showed thermal and pH stability, and remained stable after 30 days of storage at 25 °C. Thus, based on the findings of the present work, the P(MAA-co-MMA)-starch composite can be exploited as the biocompatible matrix used forl -ASNase immobilization for medical applications due to biocompatibility and biodegradability.