Growth hormone regulates the expression of UCP2 in myocytes. (August 2016)

• Record Type: Journal Article
• Authors: Futawaka, Kumi; Tagami, Tetsuya; Fukuda, Yuki; Koyama, Rie; Nushida, Ayaka; Nezu, Syoko; Imamoto, Miyuki; Kasahara, Masato; Moriyama, Kenji
• Is Part Of: Growth hormone & IGF research. Volume 29(2016)
• Page Start: 57
• Page End: 62
• Publication Date: 2016-08
• Subjects: AGHD adult growth hormone deficiency -- GH growth hormone -- GHR growth hormone receptor -- IGF-I insulin-like growth factor-I -- JAK Janus kinase -- MAPK mitogen-activated protein kinase -- PI3K phosphoinositide-3 kinase -- STAT signal transduction and activator of transcription -- UCP uncoupling protein; Adult growth hormone deficiency -- Energy metabolism -- Expression of uncoupling protein2 -- Growth hormone signaling -- Myocytes -- Obesity -- Signal transduction pathway; Growth regulators -- Periodicals; Growth -- Regulation -- Periodicals; Somatomedin -- Periodicals; Somatomedins -- Periodicals; Growth Hormone -- Periodicals; Growth Substances -- Periodicals; Croissance -- Régulation -- Périodiques; Croissance -- Régulateurs -- Périodiques; Somatotrophine -- Périodiques; Somatomédine -- Périodiques; Growth -- Regulation; Growth regulators; Electronic journals; Periodicals; Electronic journals; 612.4
• DOI: 10.1016/j.ghir.2016.04.005
• Languages: English
• ISSNs: 1096-6374

Abstract

Abstract: Objective: To determine if and how growth hormone (GH) signaling is involved in energy metabolism. Design: We used human embryonic kidney TSA201 cells, human H-EMC-SS chondrosarcoma cells, rat L6 skeletal muscle cells, and murine C2C12 skeletal muscle myoblasts to investigate GH-induced expression of uncoupling protein2 (UCP2) to the GHR/JAK/STAT5 pathway by a combination of a reporter assay, electrophoretic mobility shift assay (EMSA), real-time quantitative PCR, Western blotting. Results: We demonstrated that the regulation energy metabolism, which was hypothesized to be directly acted on by GH, involves UCP2 via activated STAT5B, a signal transducer downstream of GH. We also showed that the sequence at the − 586 'TTCnGA' may function as a novel putative consensus sequence of STAT5s. Conclusion: The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. Highlights: Signaling pathways activated by growth hormone (GH) during energy metabolism analyzed. GH found to directly enhance expression of UCP2, key protein in energy metabolism. Effect mediated via STAT5B, signal transducer downstream of GH. Results may aid development of drugs for treatment of AGHD and obesity.