The ethanolic extract of the Eclipta prostrata L. ameliorates the cognitive impairment in mice induced by scopolamine. (22nd August 2016)

• Record Type: Journal Article
• Authors: Jung, Won Yong; Kim, Haneul; Park, Ho Jae; Jeon, Se Jin; Park, Hye Jin; Choi, Hyuck Jai; Kim, Nam Jae; Jang, Dae Sik; Kim, Dong Hyun; Ryu, Jong Hoon
• Is Part Of: Journal of ethnopharmacology. Volume 190(2016)
• Page Start: 165
• Page End: 173
• Publication Date: 2016-08-22
• Subjects: ACh acetylcholine -- AChE acetylcholinesterase -- AD Alzheimer's disease -- Akt protein kinase B -- ANOVA one-way analysis of variance -- Con control -- EEEP ethanolic extract of the Eclipta prostrata L -- GSK-3β glycogen synthase kinase-3β -- LTP long-term potentiation -- TBS Theta-burst stimulating -- TTBS Tween 20/Tris-buffered saline; Eclipta prostrata l. -- Learning and memory -- Long-term potentiation -- Protein kinase b -- Glycogen synthase kinase 3β; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.06.010
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Eclipta prostrata L. (Asteraceae) has been prescribed for whole body nourishment and nervine tonic in Asia. However, the effects of E. prostrata in learning and memory have not been fully explored. Aim of the study: To scientifically elucidate the effects of E. prostrata on cognitive functions, we examined whether E. prostrata could ameliorate a cholinergic blockade-induced memory impairment, and we also investigated the effects of E. prostrata on the synaptic plasticity in the hippocampus. Materials and methods: Memory impairment was induced by scopolamine, a cholinergic muscarinic receptor antagonist. The anti-amnesic effects of the ethanolic extract of Eclipta prostrata L. (EEEP) were measured in mice by the passive avoidance, Y-maze and Morris water maze tasks. To test the effects of EEEP on synaptic plasticity, we measured long-term potentiation (LTP) in the hippocampus. We also studied several signaling molecules related to learning and memory, such as phosphorylated protein kinase B (Akt) or phosphorylated glycogen synthase kinase-3β (GSK-3β). Results: In the passive avoidance task, EEEP (50 or 100 mg/kg, p.o.) significantly ameliorated the shortened step-through latency induced by scopolamine. EEEP (100 mg/kg, p.o.) also showed significant increase in alternation behavior during the Y-maze task. In the Morris water maze task, scopolamine-induced a decrease in both the swimming time within the target zone and the number of crossings where the platform had been placed were significantly reversed by EEEP (50 or 100 mg/kg, p.o.). Moreover, EEEP (100 μg/ml) significantly enhanced hippocampal LTP without affecting basal synaptic transmission. The administration of EEEP (100 mg/kg) increased the phosphorylation levels of Akt and GSK-3β in the hippocampal region. Conclusion: These results suggest that EEEP has memory-ameliorating activity against scopolamine-induced cognitive impairment and facilitates LTP in the hippocampus. This could be, at least in part, mediated by the activation of the Akt-GSK-3β signaling pathway. Graphical abstract: